PCR Techniques

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What is PCR and where can it be used

The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million-fold in just a few hours. The PCR achieves amplification of a predetermined fragment of DNA, (the target; which can e.g. be from 100 – 1000 bp long) with the apparent disadvantage that the sequences flanking the target region must be known, the latter precludes the use of PCR from analysis of DNA regions that have not previously been studied by standard methods. 

Creators: 
Prof Estelle Venter
Language: 
English
Type: 
Other
Format: 
Other
Publisher/Source: 
Open.Michigan, University of Michigan
Audience: 
Student
License Condition: Creative Commons: Attribution 4.0  
Unit: 
Laboratory diagnostics: Molecular biology
Education Level: 
Continuing Professional Development (CPD)
Academic Year: 
2014
Instructor: 

Prof Estelle Venter

  • BSc, MSc, PhD
  • Professor: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa